摘要
目的 本实验采用超高效液相色谱法(UPLC)建立同时测定主产地(甘肃、陕西、内蒙古)黄芪药材中毛蕊异黄酮苷,芒柄花苷,槲皮素,毛蕊异黄酮,山柰酚,芒柄花素6种黄酮类成分的含量测定方法。方法 采用ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7 μm),流动相A 为0.1% 甲酸水,流动相B 为0.1% 甲酸乙腈-异丙醇(7∶3),流速为0.25 mL·min-1,梯度洗脱,检测波长254 nm,柱温30 ℃,进样量2 μL。结果 6种黄酮类成分在选定的范围内线性关系良好(r≥0.999),平均加样回收率(n=6) 在99.60%～101.2%,RSD 1.2%～2.0%。结论 本实验首次采用UPLC对黄芪中毛蕊异黄酮苷、芒柄花苷、槲皮素、毛蕊异黄酮、山柰酚、芒柄花素6种黄酮类成分进行含量测定,该方法专属性强、重复性好、稳定、可控,可作为黄芪药材质量控制的方法。
Abstract
OBJECTIVE To establish an UPLC method for simultaneous determination of six flavonoid active components (campanulin,ononin,quercetin,calycosin,kaempferol,and formononetin) of the roots of Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao collected from the main producing areas (Gansu Province,Shaanxi Province,and Inner Mongolia).METHODS Separation was performed at 30 ℃ on an ACQUITY UPLC BEH C18 column (2.1 mm ×100 mm,1.7 μm) with a gradient elution system of water containing 0.1% formic acid (mobile phase A) and acetonitrile - isopropanol (7;3) containing 0.1% formic acid (mobile phase B).The flow rate was 0.25 mL·min-1.The detection wavelength was 254 nm,and the sample injection volume was 2 μL. RESULTS The six flavonoid active components showed good linearity in the selected concentration ranges (r≥0.999),with average recoveries of 99.60%-101.2% (n=6) and RSDs of 1.2%-2.0%. CONCLUSION This study for the first time establishes an UPLC method for simultaneous determination of six flavonoid active components (campanulin,ononin,quercetin,calycosin,kaempferol,and formononetin) of the roots of Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao.This method is specific,reproducible,controllable,and can be used for the quality control of Radix Astragali.
关键词
黄芪 /
蕊异黄酮苷 /
芒柄花苷 /
槲皮素 /
含量测定 /
超高效液相色谱法
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Key words
Astragalus membranaceus /
campanulin /
ononin /
quercetin /
content determination /
UPLC
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付娟,,杨世海*,黄林芳*.
超高效液相色谱法同时测定黄芪中6种黄酮类成分的含量[J]. 中国药学杂志, 2013, 48(11): 916-919 https://doi.org/10.11669/cpj.2013.11.018
FU Juan,, YANG Shi-hai*, HUANG Lin-fang*.
Simultaneous Determination of Six Flavonoid Active Components in Radix Astragali by UPLC[J]. Chinese Pharmaceutical Journal, 2013, 48(11): 916-919 https://doi.org/10.11669/cpj.2013.11.018
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参考文献
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脚注
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基金
国家自然科学基金资助项目(30801519;81274013);国家重大科技专项创制新药(2011ZX09307-002-01;);人事部留学人员择优资助项目(2009-2011);863计划课题(2010AA023006)
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